Study on Enzymatic Extraction of Tectorigenin from Iridis Tectori Rhizoma

Abstract

Antibiotics are gradually abused in the animal feed industry, which will cause the decline of self-immunity and the emergence of drug-resistant bacteria. When people eat meat and egg products, antibiotics will be enriched in the human body, leading to adverse effects. To this end, the state limits the production of antibiotic feed and promotes the use of Chinese herbs as the best alternative to antibiotics. Chinese herbs themselves do not produce harmful substances and are a kind of green resources, Chinese herbs play a role because of the natural active ingredients in them. Enzymatic method can specifically degrade plant cell wall and release natural active ingredients through the barrier of cell wall. In this paper, the enzyme method was used to extract tectorigenin from Iridis Tectori Rhizoma. Acid protease, acid cellulase, pectinase (Ⅱ) and xylanase were selected to act on Iridis Tectori Rhizoma respectively. Using the standards of two substances, the standard curves of tectoridin and tectorigenin were determined by HPLC. The samples were extracted by enzymolysis. The extraction amounts of tectorigenin of acid protease, acid cellulase, xylanase and pectinase (Ⅱ) were 47.7 ug/g, 89.8 ug/g, 60.0 ug/g and 50.0 ug/g respectively, and the extraction amount of acid cellulase was the highest. In addition, the experiment of ultrasonic-assisted enzymolysis was carried out, and the extraction amount of acid cellulase group was increased by 91.8% compared with that of water extraction group. In tectoridin extraction experiment, the extraction effect of pectinase (Ⅱ) was the best, reaching 7.57 mg/g, which indicated that pectinase (Ⅱ) had a significant effect on cell wall hydrolysis.