Optimization of Laccase Fermentation Conditions Using Escherichia coli BL21 (DE3)

Abstract

Laccase is a kind of blue polycopper oxidase, which has been widely used in many fields of industry, such as green environmental protection, paper bleaching industry, pharmaceutical industry, sewage treatment, food industry and other fields have made a very broad application prospect. However, the low yield of laccase seriously limits the actual production and use of laccase, which cannot meet the increasing actual market demand of laccase. Therefore, this paper adopts the method of combining single factor experiment and response surface experiment to optimize the laccase fermentation conditions of Escherichia coli BL21. The software was used to analyze the experimental data and model, and the optimal fermentation process conditions for laccase fermentation production were obtained as follows: The added amount of glucose was 9.95 g, the added amount of ammonium chloride was 10.97 g, the pH was 7.52, and the predicted value of laccase activity was 7.4525 U/mL. The actual laccase activity was 7.43U/mL according to the fermentation conditions predicted by the software, which was not much different from the predicted value, proving the reliability of the software analysis. In this experiment, the laccase fermentation conditions were optimized by response surface method, which effectively improved the laccase yield, and was applied to the industrial field, which reduced the production cost and improved the economic benefits, and also provided a certain experimental basis for the subsequent use of Escherichia coli laccase fermentation.