Detection and verification of the improvement of Xiaoyao Pill on depression based on biotechnology methods

Abstract

With the continuous development and progress of science and technology, people's material life is satisfied at the same time, the pressure of social life and other aspects also comes immediately, and different people also show different reactions to the high-pressure environment. Being in the high tension and pressure of life rhythm for a long time will have a certain impact on people's physical and mental health. Depression, as a kind of disease, has been widely concerned by the society. Xiaoyao Pill, as a Chinese medicine prescription for regulating spleen and stomach weakness, has a certain improvement in the treatment of depression. In this paper, laboratory mice were selected as experimental subjects, animal models were prepared, and the depression model mice were treated with Zhizi soybean soup by gavage. After open field experiment, sugar spray experiment and tail suspension experiment, behavioral detection was carried out to evaluate the antidepressant effect of Xiaoyao Pill intervention on the depression model mice. The expression of c-fos activation in hippocampal brain region was detected by immunofluorescence, and the intervention effect of Xiaoyao Pill on pathological indexes of depression model mice was evaluated by comparing the activation amount of c-fos in hippocampal brain region between different groups. Finally, data analysis is carried out, using the research data obtained from the experiment and the body mass growth rate repeatedly measured by two-factor ANOVA. Therefore, through the detection and verification of the improvement of Xiaoyao pills on depression by biotechnological methods, we can further understand the operation and principle of biotechnological immunofluorescence technology, and provide a theoretical basis for the pharmacological effect of Xiaoyao pills on improving depression.