Metagenomic analysis of the effect of RB2 on the bacterial community composition in the rhizosphere soil of Chinese cabbage under lead pollution

Abstract

With the development of industrialization, soil heavy metal pollution is a serious problem. Our country as a large agricultural country, the impact of soil heavy metal pollution on our country should not be underestimated, so we need to find green and efficient solutions to deal with the hazards brought by the industrialization process and reduce its own defects caused by social progress. With the discovery of more and more microorganisms, microbial remediation technology rises accordingly. In recent years, excellent results have been achieved in the application of high-yielding exopolypaccharides in Marine sewage treatment, while few studies on its application to soil heavy metal pollution in China. Therefore, this project conducted high-throughput sequencing of bacterial genome DNA in rhizosphere soil of cabbage by design of a pot experiment. The influence of Marine bacteria Micrococcus antarcticus RB2 with high exopolysaccharide yield under lead (Pb) stress on the microbial community structure of the rhizosphere soil of Brassica chinensis L. was analyzed. In this study, it was found that the relative abundance of Proteobacteria and cyanobacteria, which have the ability to remove heavy metals, increased in the rhizosphere soil under the lead contamination stress of the inoculated strain RB2. Meanwhile, after the inoculated strain RB2 in the rhizosphere soil of the inoculated strain RB2, the relative abundance of Bacillus and Pseudomonas which can promote plant growth increased. Their relative abundance was higher than that of unvaccinated control group. Therefore, RB2 promoted the relative abundance of heavy metal-clearing and growth-promoting bacteria in the rhizosphere soil community under lead stress.